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Engineering a Model Cell for Rational Tuning of GPCR Signaling.

Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T
Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
123024pWS060 Pheromone responsive promoter
123025pWS053Pheromone responsive promoter
123026pWS2247Tet promoter (TetO(x7)-pLEU2m)
123027pWS1078Z3E promoter
123028pWS480LexA UAS
123029pWS481LexA UAS
123030pWS482LexA UAS
123031pWS483LexA UAS
123032pWS484LexA UAS
123033pWS485Core promoter
123034pWS494Core promoter
123035pWS491Core promoter
123036pWS487Core promoter
123037pWS493Core promoter
123038pRC246Core promoter
123039pWS486Core promoter
123040pWS498Core promoter
123041pWS496Core promoter
123042pWS497Core promoter
123043pWS495Core promoter
123044pWS067_-factor GPCR
123045pWS626P-factor GPCR
123046pWS625Adenosine GPCR
123047pWS1806Melatonin GPCR
123048pWS2119PbPeptide GPCR
123049pWS1037G_ subunit
123050pWS936G_ subunit for creating C-terminal transplants
123051pWS939Pheromone responsive transcription factor
123052pWS926LexA bacterial repressor
123053pWS931TetR bacterial repressor
123054pWS930Beta-estradiol inducible DNA binding domain
123055pWS932Pheromone responsive domain
123056pWS033Green fluorescent protein
123057pRC063Blue fluorescent protein
123058pWS1837_-factor pheromone
123059pWS788_-factor protease
123060pWS081Regulator of G protein signalling
123061pWS509Negative regulator of Fus3
123062pWS510Negative regulator of Ste12
123063pWS511Negative regulator of Ste12
123064pWS627G___ bicistronic protein
123065pWS1859Positive regulator of Ste20

Antibodies from Article