Assessment of Cas12a-mediated gene editing efficiency in plants.
Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez D
Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160572 | pUPD2 AsCas12a (GB1438) | Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a). |
| 160573 | pUPD2 LbCas12a (GB1439) | Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a). |
| 160574 | pUPD2 AtU6-26:AsCas12a_DR (GB1442) | AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes. |
| 160575 | pUPD2 AtU6-26:LbCas12a_DR (GB1443) | LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes. |
| 160576 | pUPD2 HDV (GB1444) | Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes. |
| 160594 | pUPD2 AtUBQ10 (GB2478) | UBQ10 promoter from A. thaliana for constitutive expression in plant systems. |
| 160610 | pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440) | Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter. |
| 160611 | pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441) | Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter. |