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Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation.

Chen TJ, Wang HJ, Liu JS, Cheng HH, Hsu SC, Wu MC, Lu CH, Wu YF, Wu JW, Liu YY, Kung HJ, Wang WC
Commun Biol. 2019 Mar 15;2:105. doi: 10.1038/s42003-019-0343-4. eCollection 2019. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122690pET28a-PKM2-WTExpress His-tagged PKM2 (wild type) protein in bacteria.
122691pET28a-PKM2-R399EExpress His-tagged PKM2 (R399E) protein in bacteria.
122692pET28a-PKM2-H391YExpress His-tagged PKM2 (H391Y) protein in bacteria.
122693pET28a-PKM2-G415RExpress His-tagged PKM2 (G415R) protein in bacteria.

Antibodies from Article