A Self-Actuated Cellular Protein Delivery Machine.
Ahan RE, Kirpat BM, Saltepe B, Seker UOS
ACS Synth Biol. 2019 Apr 19;8(4):686-696. doi: 10.1021/acssynbio.9b00062. Epub 2019 Mar 12.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
172886 | pET22b-PelB-6H-Ag43-160N-sfGFP | Display sfGFP protein on E. coli cell surface |
172887 | pET22b-6h-GST-TEVp | Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification |
172891 | pSecEffector | Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901 |
172892 | pSecEffector wo TEVp | atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901 |