Evaluation of site-specific homologous recombination activity of BRCA1 by direct quantitation of gene editing efficiency.
Yoshino Y, Endo S, Chen Z, Qi H, Watanabe G, Chiba N
Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1038/s41598-018-38311-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169795 | LentiCRISPRv2-scr | Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1.. |
| 169796 | LentiCRISPRv2-ACTB-C1 | Expresses Cas9 and guide RNA for ACTB gene in human |
| 169797 | LentiCRISPRv2-KLF7-1 | Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene. |
| 169798 | pBS-ACTB-C200-GFPfr1 | THe donor vector for the ACTB gene locus. |
| 169799 | pBS-KLF7-200-GFPfr1 | THe donor vector for the KLF7 gene locus. |