Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant.
Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A
Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131669 | pFUGW-GFP-2-cut reporter | Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining. |
| 131670 | pcDNA3.1(+)- e18 | Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR. |
| 131729 | pcDNA3.1(+)- e18 LOF | Expresses a loss of function mutant of an engineered variant of RAD18 ("e18") |