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Combinatorial design of chemical-dependent protein switches for controlling intracellular electron transfer

Wu B, Atkinson JT, Kahanda D, Bennett GN, Silberg JJ
BioRxiv May 23, 2019 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
131529pBW001Vector containing the Mu-BsaI-KanR transposon that can be excised with HindIII and BglII for transposition reactions
131591pBW002Vector with aTc inducible M. laminosus Fd1 without BbsI restriction sites in the vector backbone
131804pBW003Vector with aTc inducible M. laminosus Fd1 flanked by BbsI restriction sites
131805pBW004Vector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'
131806pBW005Vector with aTc inducible M. laminosus Fd1 with a C-terminal fused mRFP1
131807pBW006Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
131808pBW007Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26 with a C-terminal fused mRFP1
131809pBW008Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
131811pBW009Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31 with a C-terminal fused mRFP1
131812pBW010Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36
131814pBW011Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36 with a C-terminal fused mRFP1
131815pBW012Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55
131816pBW013Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55 with a C-terminal fused mRFP1
131817pBW014Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
131819pBW015Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56 with a C-terminal fused mRFP1
131820pBW016pET28b derrivative vector with T7-driven IPTG inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56

Antibodies from Article