Generation of genetically modified mice using SpCas9-NG engineered nuclease.
Fujii W, Ito H, Kanke T, Ikeda A, Sugiura K, Naito K
Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
131465 | pCAG-T3-SpCAS9-NG-pA | Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys |
131466 | pCAG-T3-eSpCAS9-NG-pA | Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys |