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Generation of genetically modified mice using SpCas9-NG engineered nuclease.

Fujii W, Ito H, Kanke T, Ikeda A, Sugiura K, Naito K
Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
131465pCAG-T3-SpCAS9-NG-pAExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
131466pCAG-T3-eSpCAS9-NG-pAExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Antibodies from Article