Yeast surface display platform for rapid discovery of conformationally selective nanobodies.
McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC
Nat Struct Mol Biol. 2018 Mar;25(3):289-296. doi: 10.1038/s41594-018-0028-6. Epub 2018 Feb 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131402 | pET26b_Nb.AD101 | E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor |
| 131403 | pET26b_Nb.AD102 | E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor |
| 131404 | pET26b_Nb.b201 | E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin |