CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung JK
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131299 | nCas9-UGI control for Target-AID (pRZ1047) | CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1). |
| 131300 | Target-AID (pRZ762) | CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. |
| 131311 | miniABEmax (pRZ900) | CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain). |
| 131312 | SECURE miniABEmax-K20A/R21A (pJUL1774) | CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations). |
| 131313 | SECURE miniABEmax-V82G (pJUL1828) | CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation). |
| 131314 | hA3A-BE3 (pRZ215) | CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. |
| 131315 | eA3A-BE3 (pRZ206) | CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. |
| 131316 | hAID-BE3 (pRZ352) | CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. |