Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing.
Lyu P, Javidi-Parsijani P, Atala A, Lu B
Nucleic Acids Res. 2019 Jul 12. pii: 5531787. doi: 10.1093/nar/gkz605.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
131226 | psPAX2-D64V-NC-COM | Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC) |
131227 | pSaCas9-sgRNA-Tetra-com vector | For expressing gRNA, whose Tetraloop is replaced with a com aptamer |