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Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing.

Lyu P, Javidi-Parsijani P, Atala A, Lu B
Nucleic Acids Res. 2019 Jul 12. pii: 5531787. doi: 10.1093/nar/gkz605. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
131226psPAX2-D64V-NC-COMExpressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)
131227pSaCas9-sgRNA-Tetra-com vectorFor expressing gRNA, whose Tetraloop is replaced with a com aptamer

Antibodies from Article