SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space.
Dunkler A, Rosler R, Kestler HA, Moreno-Andres D, Johnsson N
Methods Mol Biol. 2015;1346:151-68. doi: 10.1007/978-1-4939-2987-0_11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131166 | pMET17p-CCG 316 | For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant |
| 131167 | pCCG 306 | PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant |