Detecting protein-protein interactions with the Split-Ubiquitin sensor.
Dunkler A, Muller J, Johnsson N
Methods Mol Biol. 2012;786:115-30. doi: 10.1007/978-1-61779-292-2_7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131163 | pMET17p-CRU313 | For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant |
| 131164 | pFA CRU HISMX | PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant |
| 131165 | pExo84-CRU 303 | For C-terminal genomic integration of Cub-R-URA3, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant |
| 131168 | pCUP1pNuiHA kanMX CEN | for ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiae |
| 131169 | pFA CUP1p-NuiHA kanMX | PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae |
| 131170 | pFA NuiHA-loxP-nat-CUP1p | PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin |