An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation.
Renz C, Johnsson N, Gronemeyer T
BMC Biotechnol. 2013 Jul 26;13:60. doi: 10.1186/1472-6750-13-60.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
131160 | pAc-Cdc3-Cdc10 | Expression of Septin rods |
131161 | pAc-Cdc3-Cdc10-SNAP | Expression of Septin rods |
131162 | pET-Cdc12-Cdc11 | Expression of Septin rods |