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beta-Actin mRNA interactome mapping by proximity biotinylation.

Mukherjee J, Hermesh O, Eliscovich C, Nalpas N, Franz-Wachtel M, Macek B, Jansen RP
Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12863-12872. doi: 10.1073/pnas.1820737116. Epub 2019 Jun 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
131132phage - ubc - nls - 2xmcp - egfp - BirA* BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Antibodies from Article