The Crk adapter protein is essential for Drosophila embryogenesis, where it regulates multiple actin-dependent morphogenic events.
Spracklen AJ, Thornton-Kolbe EM, Bonner AN, Florea A, Compton PJ, Fernandez-Gonzalez R, Peifer M
Mol Biol Cell. 2019 Jul 18:mbcE19050302. doi: 10.1091/mbc.E19-05-0302.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131137 | pTIGER_mNeonGreen::3xFLAG::dCrk | UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion. |
| 131138 | pTIGER_tdTomato::3xFLAG::dCrk | UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion. |
| 131139 | pU6-chiRNA_dCrkgRNA1 | Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013). |
| 131140 | pU6-chiRNA_dCrkgRNA2 | Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013). |
| 131141 | pHD-dsRed-attP_dCrk_flankingHRs | Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger). |
| 131143 | pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk | Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream. |
| 131145 | pET28P_dCrk | Expresses 6XHis::dCrk (based on crk isoform RA). |
| 131146 | pGEX-6P-2_dCrk | Expresses GST::dCrk (based on crk isoform RA). |