A genetically encodable cell-type-specific protein synthesis inhibitor.
Heumuller M, Glock C, Rangaraju V, Biever A, Schuman EM
Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0468-x. doi: 10.1038/s41592-019-0468-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131008 | pTRE3G-Bi-gePSI-pCMV-AcGFP | Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter. |
| 133727 | pCAMKIIa-TetOn3G | Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter. |
| 133728 | pGFAP-TetOn3G | Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter. |
| 133729 | pCMV-FLEx-TetOn3G-3xHA-FLEx | Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase. |
| 133730 | pTRE3G-Bi-α-chain | Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter. |
| 133731 | pTRE3G-Bi-β-chain | Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter. |
| 133732 | pTRE3G-Bi-gePSI-pCamk2a-AcGFP | Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter. |
| 133733 | pTRE3G-Bi-gePSI-pGFAP-AcGFP | Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter. |