Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing.
Acharya S, Mishra A, Paul D, Ansari AH, Azhar M, Kumar M, Rauthan R, Sharma N, Aich M, Sinha D, Sharma S, Jain S, Ray A, Jain S, Ramalingam S, Maiti S, Chakraborty D
Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130966 | pET-His6-FnCas9GFP | expression of FnCas9-GFP in bacterial cells |
| 130968 | PX458-3xHA-SpCas9 | 3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA |
| 130969 | PX458-3xHA-FnCas9 | 3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA |