An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells.
Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA
Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 10.1016/j.celrep.2019.07.090.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130932 | HES7-NLuc-2A-tdTomato | Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato |
| 130933 | pCRII-Blunt-U6-HES7-STOP-gRNA | gRNA for tag human HES7 |
| 130934 | pB-TAG-NICD | PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells |
| 130935 | pB-TAG-HES7 | PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells |
| 130936 | pB-EF1a-NLuc-IRES-Puro | PiggyBac vector for constitutive NanoLuc expression in mammalian cells |