Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.
Jeng EE, Bhadkamkar V, Ibe NU, Gause H, Jiang L, Chan J, Jian R, Jimenez-Morales D, Stevenson E, Krogan NJ, Swaney DL, Snyder MP, Mukherjee S, Bassik MC
Cell Host Microbe. 2019 Oct 9;26(4):551-563.e6. doi: 10.1016/j.chom.2019.08.017. Epub 2019 Sep 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130879 | C1ORF43-GFP | Expresses human C1ORF43 with C-terminal GFP tag |
| 130880 | C1ORF43-3xFLAG | Expresses human C1ORF43 with C-terminal 3xFLAG tag |
| 130881 | C1ORF43(1-41)-3xFLAG | Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag |
| 130882 | GFP-RABIF | Expresses human RABIF with N-terminal GFP tag |
| 130883 | GFP-RAB10 | Expresses human RAB10 with N-terminal GFP tag |
| 130885 | GFP-RAB10 T23N | Expresses human RAB10 T23N mutant with N-terminal GFP tag |
| 130886 | GFP-RAB10 K154A | Expresses human RAB10 K154A mutant with N-terminal GFP tag |