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Chemogenetic Control of Nanobodies.

Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K
Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
130704pCDNA3.0_mitoLAMA-F98Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion
130705pCDNA3.0_mitoLAMA-G97Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion
130706pCDNA3.0_mitoLAMA-F98 IRES EGFPPlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP
130707pCDNA3.0_mitoLAMA-G97 IRES EGFPPlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP
130708pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarletPlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet
130709pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarletPlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet
130710pCDNA3.0_LynLAMA-F98 IRES EGFPPlasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP
130711pCDNA3.0_LynLAMA-G97 IRES EGFPPlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP
130712pCDNA3.0_nucLAMA-F98 IRES mYFPPlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP
130713pCDNA3.0_nucLAMA-G97 IRES mYFPPlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP
130714pET51b(+)_LAMA-F98 (for GFP)Plasmid encoding the bacterial expression of LAMA-F98 for GFP
130715pET51b(+)_LAMA-G97 (for GFP)Plasmid encoding the bacterial expression of LAMA-G97 for GFP
130716pET51b(+)_LAMA-N95 (for GFP)Plasmid encoding the bacterial expression of LAMA-N95 for GFP
130717pET51b(+)_EGFP-LAMA-S98 (for p24)Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein
130718pET51b(+)_SNAP-p24Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein
136618pCDNA3.0_nucLAMA-F98 IRES EGFPPlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP
136619pCDNA3.0_nucNanobody IRES EGFPPlasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP
136620pCDNA3.0_nucLAMA-G97 IRES EGFPPlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP
136621pCDNA3.0_mitoNanobody IRES EGFPPlasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP
136622pCDNA3.0_mitoNanobodyPlasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion
136623pCDNA3.0_mitoSNAPPlasmid encoding outer mitochondrial localization SNAP-Tag
136624pCDNA3.0_LynNanobody IRES EGFPPlasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP
136625pCDNA3.0_LynSNAP IRES EGFPPlasmid encoding outer inner membrane localization of SNAP-Tag and EGFP
136626pET51b(+)_EGFP_NbALFAPlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion
136627pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion
136628pET51b(+)_ALFA-tag-SNAPPlasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Antibodies from Article