An Adaptable Platform for Directed Evolution in Human Cells.
Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD
J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131685 | Wild-type AdPol vector | A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase |
| 131686 | EP-Pol vector | A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase |
| 131687 | pLVX.TRE3G.AdProt | A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor. |
| 131692 | pUC-AdEvolve-DEST | A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection. |