Single-Molecule Imaging Uncovers Rules Governing Nonsense-Mediated mRNA Decay.
Hoek TA, Khuperkar D, Lindeboom RGH, Sonneveld S, Verhagen BMP, Boersma S, Vermeulen M, Tanenbaum ME
Mol Cell. 2019 May 23. pii: S1097-2765(19)30361-2. doi: 10.1016/j.molcel.2019.05.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130697 | TPI-WT | Expresses TPI-Wildtype NMD reporter |
| 130698 | TPI-PTC160 | Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid) |
| 130699 | TPI-PTC1 | Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) |
| 130700 | Beta-Globin-WT | Expresses Beta-Globin WT NMD reporter |
| 130701 | Beta-Globin-PTC39 | Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid) |
| 131043 | HP21-TPI-PTC1 | Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR |
| 131044 | HP21-TPI-WT-no_introns | Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR |