The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti.
Matthews BJ, Younger MA, Vosshall LB
eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
130665 | Pub-mCD8:GFP-T2A-DsRed:NLS-SV40 | Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti |
130666 | 15x-QUAS-dTomato-T2A-GCaMP6s | QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A |
130667 | ppk301-T2A-QF2 HDR plasmid | Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene |