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The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti.

Matthews BJ, Younger MA, Vosshall LB
eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
130665Pub-mCD8:GFP-T2A-DsRed:NLS-SV40Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti
13066615x-QUAS-dTomato-T2A-GCaMP6sQUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A
130667ppk301-T2A-QF2 HDR plasmidPlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

Antibodies from Article