William Hancock Lab: Hancock Lab plasmids
Hancock
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129761 | K560GFP | Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays |
| 129762 | K980 | Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays |
| 129763 | KRigor980 | Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces |
| 129764 | K406 | Expresses Drosophila Kinesin-1 truncated after neck-coil at 406 |
| 129765 | K560_AviC_coBirA | Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays |
| 129766 | K560_AviN_coBirA | Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays |
| 129767 | K560GFP_17NL | Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays |
| 129768 | KIF3A406 | Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays |
| 129769 | KIF3A560GFP | Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged |
| 129770 | KSP_K560GFP | Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged |
| 129771 | KSP_K406 | Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays |
| 129772 | KSP_HNL | Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays |
| 129773 | Unc104_K560GFP | Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged |