An efficient auxin-inducible degron system with low basal degradation in human cells.
Li S, Prasanna X, Salo VT, Vattulainen I, Ikonen E
Nat Methods. 2019 Aug 26. pii: 10.1038/s41592-019-0512-x. doi: 10.1038/s41592-019-0512-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129715 | pSH-EFIRES-P-AtAFB2 | AAVS1 safe harbor targeting vector expressing AtAFB2 |
| 129716 | pSH-EFIRES-P-AtAFB2-mCherry | AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry |
| 129717 | pSH-EFIRES-P-AtAFB2-mCherry-weak NLS | AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS |
| 129718 | pSH-EFIRES-B-AtAFB2-mCherry | AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry |
| 129719 | pSH-EFIRES-B-Seipin-miniIAA7-mEGFP | AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin |
| 129720 | pSH-EFIRES-P-Seipin-miniIAA7-mCherry | AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin |
| 129721 | pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I | AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin |
| 129722 | pSH-EFIRES-P-Seipin-miniIAA7-3XFlag | AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin |
| 129725 | pCas9/VRQR-sgRNA (BbsI) | Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM |
| 129726 | pCas9-sgAAVS1-1 | Expressing SpCas9 and sgAAVS1-1 |
| 129727 | pCas9-sgAAVS1-2 | Expressing SpCas9 and sgAAVS1-2 |