Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor.
Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM
Genes Dev. 2019 Jun 1;33(11-12):718-732. doi: 10.1101/gad.320168.118. Epub 2019 Apr 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129710 | pMS22 | Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion |
| 229454 | pVF17 | Expresses IraM with His-tag |
| 229455 | pVF14 | Expresses IraD with His-tag |
| 229456 | pVF18 | Expresses IraD (aa17-130) with His-tag |
| 229457 | pVF25 | Expresses IraD with R67A mutation with His-tag |
| 229458 | pVF26 | Expresses IraD with Q84A mutation with His-tag |
| 229459 | pVF40 | Expresses IraD with R70A mutation with His-tag |
| 229461 | pMS12 | Expresses IraD with W64R mutation with His-tag |
| 229462 | pMS18 | Expresses IraD with Q68A mutation with His-tag |
| 229463 | pBD1 | Expresses RssB |
| 229464 | pMS11 | Expresses RssB with His Tag |
| 229465 | pVF16 | Expresses RssB with D58P mutation |
| 229467 | pVF42 | Expresses RssB with S29C & E229C mutation |
| 229468 | pVF31 | Expresses RssB with Q254A mutation |
| 229469 | pVF28 | Expresses RssB with D104A mutation |
| 229470 | pMS23 | Expresses RssB with D104A mutation with His-tag |
| 229471 | pMS26 | Expresses RssB with D104N mutation with His-tag |
| 229472 | pVF30 | Expresses RssB with R115A mutation |
| 229473 | pVF29 | Expresses RssB with N256A mutation |
| 229474 | pMS13 | Expresses RssB with Q254A & N256A mutation |
| 229475 | pMS19 | Expresses RssB with E135A, E136A, & E137A mutation |
| 229476 | pMS21 | Expresses RssB with E135A, E136A, & E137A mutation with His-tag |
| 229477 | pVF53 | expresses RssBE229C |