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Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue.

Tetone LE, Friedman LJ, Osborne ML, Ravi H, Kyzer S, Stumper SK, Mooney RA, Landick R, Gelles J
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1081-E1090. doi: 10.1073/pnas.1616525114. Epub 2017 Jan 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129689pET15b-GBexpress E. coli GreB
129690pET15b-GBdmexpress E. coli GreB E82C/C68S
129691pET15b-GreBdm-D41Nexpress E. coli GreB E82C/C68S/D41N

Antibodies from Article