CRISPR-Cas12b enables efficient plant genome engineering.
Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, Qi Y
Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129670 | pYPQ290 | Gateway entry clone for CRISPR-Cas12b systems |
| 129671 | pYPQ291 | Gateway entry clone for CRISPR-Cas12b systems |
| 129672 | pYPQ292 | Gateway entry clone for CRISPR-Cas12b systems |
| 129673 | pYPQ290-D570A | Gateway entry clone for CRISPR-Cas12b systems |
| 129674 | pYPQ290-D977A | Gateway entry clone for CRISPR-Cas12b systems |
| 129675 | pYPQ290-E848A | Gateway entry clone for CRISPR-Cas12b systems |
| 129676 | pYPQ291-D573A | Gateway entry clone for CRISPR-Cas12b systems |
| 129677 | pYPQ291-D951A | Gateway entry clone for CRISPR-Cas12b systems |
| 129678 | pYPQ291-E827A | Gateway entry clone for CRISPR-Cas12b systems |
| 129679 | pYPQ292-D570A | Gateway entry clone for CRISPR-Cas12b systems |
| 129680 | pYPQ292-D977A | Gateway entry clone for CRISPR-Cas12b systems |
| 129681 | pYPQ292-E848A | Gateway entry clone for CRISPR-Cas12b systems |
| 129682 | pYPQ290-D570A-SRDX | Gateway entry clone for CRISPR-Cas12b systems |
| 129683 | pYPQ291-D573A-SRDX | Gateway entry clone for CRISPR-Cas12b systems |
| 129684 | pYPQ292-D570A-SRDX | Gateway entry clone for CRISPR-Cas12b systems |
| 129685 | pYPQ141-ZmUbi-RZ-Aac | (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter |
| 129686 | pYPQ141-ZmUbi-RZ-Bth | (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter |
| 136372 | pYPQ141-ZmUbi-RZ-Aa1.2.3 | Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used |
| 136373 | pYPQ141-ZmUbi-RZ-Aa1.2 | Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used |
| 136374 | pYPQ141-ZmUbi-RZ-Aa3.8.3 | Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used |
| 136375 | pYPQ141-ZmUbi-RZ-Aa3.8.4 | Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used |
| 136376 | pYPQ141-ZmUbi-RZ-Aa3.8 | Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used |
| 136377 | pYPQ141-ZmUbi-RZ-Aac.3 | Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used |
| 136378 | pYPQ141-ZmUbi-RZ-Bh | Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing |
| 136379 | pYPQ239A (dFnCas12a)-TV | CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system |
| 136380 | pYPQ292 (AaCas12b)-D570A-TV | CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system |
| 136381 | pYPQ292 (AaCas12b)-D570A-TV-MS2-TV | CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein |
| 136382 | pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR | CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein |
| 136383 | pYPQ293 (BhCas12b_v4) | Gateway entry clone for BhCas12b_v4 |