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FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics.

Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata S, Matsuda M
Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 10.1038/s41592-019-0541-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129651pCAGGS_2paCRY22P-activatable CRY2
129652pCAGGS_2paCRY2(D387A)2P-activatable CRY2 with the FAD-disrupting mutation
129653pT2ADWpuro_2paRAFFor generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1
129654pT2ADW_2paRAFFor generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1
129655pCAGGS_2paLINX2P-activatable light-inducible nuclear exporter

Antibodies from Article