A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo.

Zhao N, Kamijo K, Fox PD, Oda H, Morisaki T, Sato Y, Kimura H, Stasevich TJ
Nat Commun. 2019 Jul 3;10(1):2947. doi: 10.1038/s41467-019-10846-1. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
129590	pCMV-15F11-HA-mEGFP	The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
129591	pCMV-15F11-HA-mCh	The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
129592	pCMV-15F11-HA-Halo	The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
129593	pET23b-15F11-HA-mEGFP-6xHis	The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
129594	pET23b-2E2-HA-mEGFP-6xHis	The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
129595	pCMV-2E2-HA-mEGFP	The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
129596	pCMV-2E2-HA-mCh	The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.