Proximity RNA Labeling by APEX-Seq Reveals the Organization of Translation Initiation Complexes and Repressive RNA Granules.
Padron A, Iwasaki S, Ingolia NT
Mol Cell. 2019 Aug 22;75(4):875-887.e5. doi: 10.1016/j.molcel.2019.07.030.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129640 | pcDNA5/FRT/TO APEX2-GFP | Express APEX2-GFP fusion in mammalian cells |
| 129641 | pcDNA5/FRT/TO C1-APEX2 | Express ER-localized C1-APEX2 fusion in mammalian cells |
| 129642 | pcDNA5/FRT/TO CBX1-APEX2 | Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells |
| 129644 | pcDNA5/FRT/TO APEX2-eIF4E1 | Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells |
| 129645 | pHis APEX2-eIF4A1 | Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria |