Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii.
Walker MP, Lindner SE
J Biol Chem. 2019 Jun 14;294(24):9555-9566. doi: 10.1074/jbc.RA118.007121. Epub 2019 May 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129520 | pSL1166 | pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation |
| 129521 | pSL1211 | pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation |
| 129522 | pSL1394 | pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation |
| 129523 | pSL1433 | pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation |
| 129525 | pSL1045 | pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation |
| 129526 | pSL1103 | pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation |
| 129527 | pSL1315 | pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation |