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Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.

Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht CG, Shi J, Querard J, Pan B, Rossignol J, Moncoq K, Morellet N, Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien L, Gautier A
Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. doi: 10.1073/pnas.1513094113. Epub 2015 Dec 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
130719pAG87 FASTExpresses His-tagged FAST (also called YFAST) in bacteria
130720pAG104 FASTExpresses FAST (also called YFAST) in mammalian cells
130721pAG106 Lyn11-FASTExpresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells
130722pAG109 H2B-FASTExpresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells
130723pAG156 Mito-FASTExpresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

Antibodies from Article