Structural and functional characterization of G protein-coupled receptors with deep mutational scanning.
Jones EM, Lubock NB, Venkatakrishnan AJ, Wang J, Tseng AM, Paggi JM, Latorraca NR, Cancilla D, Satyadi M, Davis JE, Babu MM, Dror RO, Kosuri S
Elife. 2020 Oct 21;9. pii: 54895. doi: 10.7554/eLife.54895.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129788 | Bxb1Donor_WT_ADRB2_LucReporter | encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene |
| 129789 | Bxb1Donor_W99A_ADRB2_LucReporter | encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene |
| 129790 | Bxb1Donor_N51L_ADRB2_LucReporter | encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene |
| 129793 | Bxb1Donor_G315T_ADRB2_LucReporter | encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene |
| 129794 | Bxb1Donor_G102H_ADRB2_LucReporter | encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene |