Seipin Facilitates Triglyceride Flow to Lipid Droplet and Counteracts Droplet Ripening via Endoplasmic Reticulum Contact.
Salo VT, Li S, Vihinen H, Holtta-Vuori M, Szkalisity A, Horvath P, Belevich I, Peranen J, Thiele C, Somerharju P, Zhao H, Santinho A, Thiam AR, Jokitalo E, Ikonen E
Dev Cell. 2019 Jun 6. pii: S1534-5807(19)30388-0. doi: 10.1016/j.devcel.2019.05.016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129412 | pGL3-sgACSL3.C-Cas9-P2A-Puro | Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus |
| 129413 | pGL3-Basic-ACSL3-sfGFP(C) HDR template | HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP |
| 129415 | pGL3-Basic-Rab18-sfGFP(N) HDR template | HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP |
| 129416 | pSH-EFIRES-P-GFP(1-10)opti | Expressing codon-optimized GFP(1-10) fragment in human cells |
| 129418 | pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro | Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus |