Live imaging of mRNA using RNA-stabilized fluorogenic proteins.
Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR
Nat Methods. 2019 Sep;16(9):862-865. doi: 10.1038/s41592-019-0531-7. Epub 2019 Aug 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129401 | CMV-mCherry-(F30-2xPepper)10 | mCherry mRNA reporter tagged with (F30-2xPepper)10 |
| 129402 | miniCMV-(mNeonGreen)4-tDeg | (mNeonGreen)4-tDeg fluorogenic protein |
| 129403 | CMV-CytERM-mCherry-(F30-2xPepper)10 | CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10 |
| 129404 | UbC-(mNeonGreen)4-tDeg | (mNeonGreen)4-tDeg fluorogenic protein |
| 129405 | pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2) | circular Pepper RNA |
| 129406 | pAV-U6+27-Tornado-F30-TAR Variant-1 | circular TAR Variant-1 |