Skip to main content
Addgene

Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins.

Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R
J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 10.1074/jbc.AC119.009977. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129289pCMV 3xFLAG-LC3B Q116P G120Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells
129290pDEST-CMV GFP-LC3B Q116P G120Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells
129291pDONR223 LC3B Q116P G120Gateway entry clone encoding human deconjugation-resistant LC3B
129292pDONR223 ATG3 WTGateway entry clone encoding wild-type human ATG3 with a stop codon
129293pDONR223 ATG3 C264AGateway entry clone encoding human ATG3 C264A (catalytic inactive)
129294pDONR223 ATG3 K243RGateway entry clone encoding human ATG3 K243R
129295pDONR223 ATG3 allKRGateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine
129296pCMV 3xFLAG-ATG3 WTExpresses 3xFLAG-ATG3 WT in mammalian cells
129297pCMV 3xFLAG-ATG3 C264AExpresses 3xFLAG-ATG3 C264A in mammalian cells
129298pCMV 3xFLAG-ATG3 allKRExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells
129299pCMV 3xFLAG-ATG3 1-13 KRExpresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells
129300pCMV 3xFLAG-ATG3 14-22 KRExpresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells
129301pCMV 3xFLAG-ATG3 K243RExpresses pCMV 3xFLAG-ATG3 K243R in mammalian cells
129302pCMV 3xFLAG-ATG3 K295RExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

Antibodies from Article