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Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph.

Bennett RK, Gonzalez JE, Whitaker WB, Antoniewicz MR, Papoutsakis ET
Metab Eng. 2018 Jan;45:75-85. doi: 10.1016/j.ymben.2017.11.016. Epub 2017 Dec 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129104pETM6_Ptrc_BsMdh_BmHps_BmPhiExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus
129105pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdcExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

Antibodies from Article