Targeted De-Methylation of the FOXP3-TSDR Is Sufficient to Induce Physiological FOXP3 Expression but Not a Functional Treg Phenotype.
Kressler C, Gasparoni G, Nordstrom K, Hamo D, Salhab A, Dimitropoulos C, Tierling S, Reinke P, Volk HD, Walter J, Hamann A, Polansky JK
Front Immunol. 2021 Jan 7;11:609891. doi: 10.3389/fimmu.2020.609891. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129025 | pSpdCas9-huTET1CD-T2A-GFP (PX458) | targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA |
| 129026 | pSpdCas9-hudTET1CD-T2A-GFP (PX458) | inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA |
| 129027 | pSpdCas9-huTET1CD-T2A-mCherry(PX458) | targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA |
| 129028 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458) | inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA |
| 129029 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR |
| 129030 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR |
| 129031 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR |
| 129032 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR |
| 129033 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-5 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting human TSDR |
| 129034 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR |
| 129035 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR |
| 129036 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR |
| 129037 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR |
| 129038 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR |
| 129039 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR |
| 129040 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12 | targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting human TSDR |
| 129041 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR |
| 129042 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR |
| 129043 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR |
| 129044 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR |
| 129045 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR |
| 129046 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR |
| 129047 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR |
| 129048 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR |
| 129049 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR |
| 129050 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR |
| 129051 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR |
| 129052 | pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12 | inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR |
| 129053 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR |
| 129054 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-2 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting mouse TSDR |
| 129055 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-3 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting mouse TSDR |
| 129056 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-4 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting mouse TSDR |
| 129057 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-5 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting mouse TSDR |
| 129058 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-6 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting mouse TSDR |
| 129059 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-7 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting mouse TSDR |
| 129060 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-8 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting mouse TSDR |
| 129061 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDR |
| 129062 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-10 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting mouse TSDR |
| 129063 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-11 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting mouse TSDR |
| 129064 | pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-12 | targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting mouse TSDR |