An improved method for circular RNA purification using RNase R that efficiently removes linear RNAs containing G-quadruplexes or structured 3' ends.
Xiao MS, Wilusz JE
Nucleic Acids Res. 2019 Jul 3. pii: 5527976. doi: 10.1093/nar/gkz576.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129020 | pcDNA3.1(+) eGFP | Expresses eGFP |
| 129021 | pcDNA3.1(+) eGFP PPP1R8 G4 | Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR |
| 129022 | pcDNA3.1(+) eGFP SH3BP5 G4 | Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR |
| 129023 | pcDNA3.1(+) eGFP EML2 G4 | Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR |