Restoration of wild-type motility to flagellin-knockout Escherichia coli by varying promoter, copy number and induction strength in plasmid-based expression of flagellin.

Thomson NM, Pallen MJ
Curr Res Biotechnol. 2020 Nov;2:45-52. doi: 10.1016/j.crbiot.2020.03.001. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
128857	pD441_WT_hi	High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter
128856	pJ291_WT_med	Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter
128855	pJ291_WT_low	Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter
128853	pJ211_rha_WT_hi	High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter
128852	pJ211_rha_WT_med	Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter
128851	pJ211_rha_WT_low	Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter
128850	pJ211_WT_hi	High copy number plasmid for expression of E. coli flagellin from its natural promoter
128849	pJ211_WT_med	Medium copy number plasmid for expression of E. coli flagellin from its natural promoter
128848	pJ211_WT_low	Low copy number plasmid for expression of E. coli flagellin from its natural promoter