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Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.

Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, Kaplan T, Buganim Y
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128827FUW-TetO-Gata6dox-dependent expression of mouse Gata6
128828FUW-TetO-Sox17dox-dependent expression of mouse Sox17
128829FUW-TetO-Cdx2dox-dependent expression of mouse Cdx2
128830FUW-TetO-Klf5dox-dependent expression of mouse Klf5
128831FUW-TetO-Sox15dox-dependent expression of mouse Sox15
128833pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLSKnock-in of nuclear EYFP into mouse Elf5 locus
128834pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpAKnock-in of tdTomato into mouse Utf1 locus
128835pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpAKnock-in of TagBFP into mouse Esrrb locus
128836pX330-Elf5 gRNAgRNA for targeting mouse Elf5 loci using CRISPR-cas technique
128841pX330-Esrrb gRNAgRNA for targeting mouse Esrrb locus using CRISPR-cas technique
128842pX330-Utf1 gRNAgRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Antibodies from Article