Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors and Sources to Regulate Spontaneous Release.
Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128812 | pFSW Doc2alpha WT | To make virus expressing Doc2alpha WT |
| 128813 | pFSW Doc2alpha D291N mutant | To make virus expressing Doc2alpha D291 mutant |
| 128814 | pFSW Doc2beta WT | To make virus expressing Doc2beta WT |
| 128815 | pFSW Doc2beta D303N mutant | To make virus expressing Doc2beta D303N mutant |
| 128816 | pFSW Doc2beta 6X mutant | To make virus expressing Doc2beta 6X mutant |
| 128817 | pFSW Doc2alpha Halotag | To make virus expressing Doc2alpha Halotag |
| 128818 | pFSW Doc2beta Halotag | To make virus expressing Doc2beta Halotag |
| 128819 | pEF Doc2alpha WT GFP | To visualize Doc2alpha WT in PC12 cells |
| 128820 | pEF Doc2beta WT GFP | To visualize Doc2beta WT in PC12 cells |
| 128821 | pEF Doc2alpha D291N mutant GFP | To visualize Doc2alpha D291N mutant in PC12 cells |
| 128822 | pEF Doc2beta D303N mutant GFP | To visualize Doc2beta D303N mutant in PC12 cells |
| 128823 | pEF Doc2beta 6X mutant GFP | To visualize Doc2beta 6X mutant in PC12 cells |
| 188980 | pEF Synaptophysin-mRuby | Synaptic buton marker |
| 188981 | pEF Synaptophysin-GCamp6s | Presynaptic fluorescent Ca2+ indicator |