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Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting.

Lyon K, Aguilera LU, Morisaki T, Munsky B, Stasevich TJ
Mol Cell. 2019 Jul 11;75(1):172-183.e9. doi: 10.1016/j.molcel.2019.05.002. Epub 2019 Jun 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128610Multi-frame tagIn the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.
128611smHA multi-frame taga spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

Antibodies from Article