Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments.
Benhalevy D, Anastasakis DG, Hafner M
Nat Methods. 2018 Dec;15(12):1074-1082. doi: 10.1038/s41592-018-0220-y. Epub 2018 Nov 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107596 | pEXP(V5-APEX2-NES) | Expresses V5-APEX2-NES in mammalian cells |
| 107597 | pEXP(V5_H2B_APEX2) | Expresses V5_H2B_APEX2 in mammallian cells |
| 168207 | pDEST(frt)(ATG_V5-APEX2_noSTOP) | Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection |