Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity in mRNA Decoding.
Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis LD, Tanenbaum ME
Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128602 | Nb-gp41-GFP (MoonTag-Nb-GFP) | Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array). |
| 128603 | Nb-gp41-Halo (MoonTag-Nb-Halo) | Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array). |
| 128604 | 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter) | Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR. |
| 128605 | 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter) | Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins. |
| 128606 | 12xMoonTag-12xSunTag-kif18b-24xPP7 | Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR. |
| 128607 | MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter) | Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR. |
| 128608 | SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter) | Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR. |
| 128609 | uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter) | Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR. |