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Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding.

Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 10.1016/j.cell.2019.10.035. Epub 2019 Nov 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128590pRSF-1b-S2Expresses tagless S2 ribosomal protein in E.coli
128591pRSF-1b-S3Expresses tagless S3 ribosomal protein in E.coli
128592pRSF-1b-S7-S83C/Δ157-178Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli
128594pRSF-1b-S10Expresses tagless S10 ribosomal protein in E.coli
128596pRSF-1b-S14Expresses tagless 30S ribosomal subunit protein S14 in E.coli
128597pRSF-1b-S19-T48CExpresses tagless S19-T48C mutant 30S ribosomal protein in E.coli
132948pET-S9Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli
132949pRSF-1b-S3-M129CExpresses tagless S3-M129C mutant ribosomal protein in E.coli
133048pRSF-1b-S15-T79CExpresses tagless S15-T79C mutant ribosomal protein in E.coli

Antibodies from Article