RAC1(P29S) Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance.
Lionarons DA, Hancock DC, Rana S, East P, Moore C, Murillo MM, Carvalho J, Spencer-Dene B, Herbert E, Stamp G, Damry D, Calado DP, Rosewell I, Fritsch R, Neubig RR, Molina-Arcas M, Downward J
Cancer Cell. 2019 Jul 8;36(1):68-83.e9. doi: 10.1016/j.ccell.2019.05.015. Epub 2019 Jun 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128580 | pBABE-hygro-2xMyc-Rac1-WT | For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus. |
| 128581 | pBABE-hygro-2xMyc-Rac1-P29S | For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus. |
| 128582 | pBABE-hygro-2xMyc-Rac1-Q61L | For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus. |
| 128583 | pLZRS-ER-Rac1-P29S-shortlinker | For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT |
| 128584 | pLZRS-ER-Rac1-Q61L-shortlinker | For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT |