Recombinant production of human alpha2-macroglobulin variants and interaction studies with recombinant G-related alpha2-macroglobulin binding protein and latent transforming growth factor-beta2.
Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas T
Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128492 | pIE-hα2M-H6 | Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag. |
| 128493 | pIE-N-hα2M-H6 | Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag. |
| 128494 | pIE-C-hα2M-H6 | Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag. |
| 128495 | pS6-hα2M-H6 | Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag. |
| 128496 | pS6-N-hα2M-H6 | Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag. |
| 128497 | pS6-C-hα2M-H6 | Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag. |
| 128498 | pCri-H6-TEV-GRAB | Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV. |
| 128499 | pCri-H6-TEV-GRAB-STREP | Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag. |
| 128500 | pIE-TGFB2-H6 | Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag. |
| 128502 | pS6-TGFB2-H8 | Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag. |
| 128503 | pS6-TGFB2-STREP | Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag. |