Recombinant production of human alpha2-macroglobulin variants and interaction studies with recombinant G-related alpha2-macroglobulin binding protein and latent transforming growth factor-beta2.

Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas T
Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
128503	pS6-TGFB2-STREP	Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.
128502	pS6-TGFB2-H8	Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.
128500	pIE-TGFB2-H6	Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.
128499	pCri-H6-TEV-GRAB-STREP	Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.
128498	pCri-H6-TEV-GRAB	Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.
128497	pS6-C-hα2M-H6	Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.
128496	pS6-N-hα2M-H6	Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.
128495	pS6-hα2M-H6	Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.
128494	pIE-C-hα2M-H6	Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.
128493	pIE-N-hα2M-H6	Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.